Bio Tech-Talks by Dr. Abhimannue

  Light Microscopy for Observing Bacterial Morphology Using Permanent Slides   Objective: To observe and identify the morphological characteristics of bacteria (such as shape and arrangement) using a compound light microscope and permanent stained slides .   Principle: Light microscopy uses visible light to magnify images of small specimens. In bacterial studies, staining techniques (e.g., Gram staining) enhance contrast, allowing for better observation of morphology —such as coccus , bacillus , spirillum , and their arrangements (e.g., diplo-, strepto-, staphylo-).   Materials Required: ·          Compound light microscope ·          Permanent bacterial slides ·          Immersion oil (for 100× objective) ·          Lens cleaning tissue ·        ...

  SEPARATION OF COMPOUNDS FROM A MIXTURE USING TLC Objective: To separate and identify individual compounds from a given mixture using Thin Layer Chromatography (TLC) technique. Principle: Thin Layer Chromatography is based on the differential adsorption of compounds on a stationary phase and their differential solubility in a mobile phase. ·          The stationary phase is a thin layer of adsorbent (e.g., silica gel or alumina) on a glass or plastic plate. ·          The mobile phase is a solvent or solvent mixture that moves up the plate via capillary action. ·          Compounds move at different rates depending on their polarity and affinity to the stationary and mobile phases. Materials Required: ·          TLC plate (silica gel-coated) ·          Developi...