Bio Tech-Talks by Dr. Abhimannue

  Experiment No: 3 - DNA Extraction from a Plant Sample Objective: To extract and observe DNA from a plant sample, such as spinach, strawberries, or bananas, using a simple laboratory procedure. Materials: Fresh plant sample (e.g., spinach leaves, strawberries, or bananas) Mortar and pestle SDS Salt (sodium chloride) Distilled water Ethanol (chilled in a freezer) Cheesecloth Test tubes or small beakers Glass rod or wooden skewer (for DNA spooling) Measuring Cylinder Procedure: 1. Preparation of the Plant Sample: Homogenization: Place a small amount of the plant sample (about 2-3 grams) into a mortar. Add a small amount of distilled water (about 10 ml) and a pinch of salt (about 1 gram). Grind the sample with the pestle until a smooth paste is formed. Transfer: Pour the homogenized mixture into a test tube or small beaker. 2. Lysis of Plant Cells: Add Detergent:...

  EXPERIMENT NO: 6 TITLE: Preparation of Single Cell Suspension from Chicken Liver (Primary Cell Culture) Objective: To prepare a single cell suspension from chicken liver tissue for primary cell culture, which involves the isolation, disaggregation, and suspension of cells from the liver tissue. Materials Required: Chicken liver tissue (freshly obtained) Phosphate-Buffered Saline (PBS) Enzymes: Collagenase, Trypsin-EDTA Sterile Petri dishes Sterile scissors and forceps Scalpel Sterile gauze Tissue culture media (e.g., DMEM, RPMI-1640) Fetal Bovine Serum (FBS) Centrifuge Sterile conical centrifuge tubes (15 mL and 50 mL) Pipettes and sterile pipette tips Cell strainer (70 µm or 100 µm mesh) Hemocytometer Incubator (37°C, 5% CO2) Personal Protective Equipment (PPE): Lab coat, gloves, safety goggles Procedure: 1. Collection of Chicken Liver Tissue: Tissue Collection: Obtain a fresh chicken liver and immediately ...

  CELL CYCLE CHECKPOINTS   Overview: Cell cycle checkpoints are critical control mechanisms that ensure the proper progression of the cell cycle. These checkpoints prevent cells from proceeding to the next phase until certain conditions are met, thereby safeguarding the integrity of the cell's genetic material and ensuring that cell division occurs accurately. Main Phases of the Cell Cycle: G1 Phase (Gap 1): Cell grows and prepares for DNA replication. S Phase (Synthesis): DNA replication occurs. G2 Phase (Gap 2): Cell prepares for mitosis. M Phase (Mitosis): Cell divides into two daughter cells. G0 Phase: A resting phase where cells may exit the cell cycle temporarily or permanently. Key Cell Cycle Checkpoints: G1 Checkpoint (Restriction Point): Location: Occurs at the end of the G1 phase before entry into the S phase. Function: This checkpoint assesses whether the cell h...