DNA Extraction from a Plant Sample
Experiment No: 3 - DNA Extraction from a Plant Sample Objective: To extract and observe DNA from a plant sample, such as spinach, strawberries, or bananas, using a simple laboratory procedure. Materials: Fresh plant sample (e.g., spinach leaves, strawberries, or bananas) Mortar and pestle SDS Salt (sodium chloride) Distilled water Ethanol (chilled in a freezer) Cheesecloth Test tubes or small beakers Glass rod or wooden skewer (for DNA spooling) Measuring Cylinder Procedure: 1. Preparation of the Plant Sample: Homogenization: Place a small amount of the plant sample (about 2-3 grams) into a mortar. Add a small amount of distilled water (about 10 ml) and a pinch of salt (about 1 gram). Grind the sample with the pestle until a smooth paste is formed. Transfer: Pour the homogenized mixture into a test tube or small beaker. 2. Lysis of Plant Cells: Add Detergent:...