Biotechnology Talks by Anu Abhimannue

  EXPERIMENT NO: 6 TITLE: Preparation of Single Cell Suspension from Chicken Liver (Primary Cell Culture) Objective: To prepare a single cell suspension from chicken liver tissue for primary cell culture, which involves the isolation, disaggregation, and suspension of cells from the liver tissue. Materials Required: Chicken liver tissue (freshly obtained) Phosphate-Buffered Saline (PBS) Enzymes: Collagenase, Trypsin-EDTA Sterile Petri dishes Sterile scissors and forceps Scalpel Sterile gauze Tissue culture media (e.g., DMEM, RPMI-1640) Fetal Bovine Serum (FBS) Centrifuge Sterile conical centrifuge tubes (15 mL and 50 mL) Pipettes and sterile pipette tips Cell strainer (70 µm or 100 µm mesh) Hemocytometer Incubator (37°C, 5% CO2) Personal Protective Equipment (PPE): Lab coat, gloves, safety goggles Procedure: 1. Collection of Chicken Liver Tissue: Tissue Collection: Obtain a fresh chicken liver and immediately ...

  CELL CYCLE CHECKPOINTS   Overview: Cell cycle checkpoints are critical control mechanisms that ensure the proper progression of the cell cycle. These checkpoints prevent cells from proceeding to the next phase until certain conditions are met, thereby safeguarding the integrity of the cell's genetic material and ensuring that cell division occurs accurately. Main Phases of the Cell Cycle: G1 Phase (Gap 1): Cell grows and prepares for DNA replication. S Phase (Synthesis): DNA replication occurs. G2 Phase (Gap 2): Cell prepares for mitosis. M Phase (Mitosis): Cell divides into two daughter cells. G0 Phase: A resting phase where cells may exit the cell cycle temporarily or permanently. Key Cell Cycle Checkpoints: G1 Checkpoint (Restriction Point): Location: Occurs at the end of the G1 phase before entry into the S phase. Function: This checkpoint assesses whether the cell h...

  EXPERIMENT NO: TITLE: PREPARATION OF TISSUE CULTURE MEDIA   Introduction: Animal tissue culture media are specially formulated solutions used to grow and maintain animal cells or tissues in vitro (outside the organism in a controlled environment). These media provide essential nutrients, growth factors, and environmental conditions needed for cell survival, growth, and function. Here are some key components and commonly used types of animal tissue culture media:   Basic Components of Tissue Culture Media: Amino Acids: Essential for protein synthesis. Vitamins: Act as coenzymes and antioxidants. Inorganic Salts: Maintain osmotic balance and membrane potential. Carbohydrates (e.g., glucose): Primary energy source for cells. Fatty Acids and Lipids: Used for membrane formation and energy. Serum: Provides growth factors, hormones, and other proteins. Buffering Agents (e.g., bicarbonate, HEPES): Maintain pH stability. Antibiotics: ...